Figure 5. Accumulation of tegument-like materials along the cytosolic face of the TGN-derived vacuoles.

Ultrathin sections of Epon-embedded cells. A) Numerous vacuoles were observed around the Golgi apparatus (g), and their membrane domains were modified with electron-dense tegument-like materials (arrowheads). Note the microtubule-organizing center (boxed area and the inset). B) Large tubulo-vacuoles (asterisk) were located in or near the TGN (tgn) and often contained enveloped virions, while clathrin-coated vesicles (arrow) budding from such tubulo-vacuoles were observed. Arrowheads indicate electron-dense materials resembling tegument. Moreover, inwardly budding profiles of the membrane of such tubulo-vacuoles (open arrow) with internal vesicles (open arrowhead) were observed. C and D) Examples of vacuoles with multiple membrane domains decorated with electron-dense tegument-like materials (arrowheads). E) An example of the vacuoles with budding clathrin-coated vesicles and multiple membrane domains with electron-dense tegument-like materials (arrow). F) L-particle (arrow) was found near plasma membrane. Scale bars: 0.3 μm.