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. 2008 Oct;10(10):1999–2011. doi: 10.1111/j.1462-5822.2008.01183.x

Fig. 2.

Fig. 2

Immunoblot analysis with anti-PfSUMO and anti-FLAG antibodies. Total parasite extracts from mid to late trophozoites (30–32 h post invasion) of 3D7 and 3D7 PfSUMO-FLAG tag expressing parasites were separated by 4–12% SDS-PAGE and immunoblotted with anti-PfSUMO pre-immune, anti-PfSUMO sera and anti-FLAG antibodies. Arrows indicate multiple bands corresponding to probable SUMO substrates identified in 3D7 and PfSUMO tagged parasite extracts by anti-PfSUMO and anti-FLAG antibodies respectively. Extracts from uninfected red blood cells were separated by 4–12% SDS-PAGE and immunoblotted with anti-PfSUMO pre-immune, anti-PfSUMO sera and anti-FLAG antibodies.