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. 2008 Oct;10(10):1999–2011. doi: 10.1111/j.1462-5822.2008.01183.x

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© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 3 — PfSUMO immunofluorescence assays throughout the blood stage cycle.

A. Reactivity of anti-PfSUMO sera on wild-type 3D7 strain parasites.

B. Reactivity of anti-FLAG (tag) antibody on PfSUMO-FLAG-tagged parasites. Ring stage 10–14 h, trophozoite stage 26–30 h, schizont stage 40–46 h post invasion.

C. Comparison of IFA pattern obtained by anti-PfSUMO and anti-FLAG antibodies on PfSUMO-FLAG-tagged parasites (trophozoites). Size bar corresponds to 1 μm. Nuclear staining by DAPI in blue.