Table 1.
Putative SUMO substrates identified by LC-MS/MS analysis of Plasmodium falciparum proteins.
| Gene IDa | Protein name | Putative function | Location | No. of peptides |
|---|---|---|---|---|
| Proteins identified as sumoylation substrates by two or more peptides | ||||
| PFD0415c | Hypothetical Protein | ND | ND | 6 |
| Hypothetical Protein | ND | ND | 6 | |
| PFC0140c | N-ethylmaleimide-sensitive fusion protein, putative | Vesicular trafficking | IE cytoplasmic vesicular structures | 6 |
| PF14_0326* | Dynein-related AAA-type ATPase | Invasion | Merozoite | 5 |
| PFL1525c* | Pre-mRNA splicing factor RNA helicase, putative | Splicing | ND | 3 |
| PF14_0314 | Chromatin assembly factor 1 p55 subunit | Transcription factor | ND | 2 |
| PF14_0348 | ATP-dependent Clp protease proteolytic subunit, putative | ND | ND | 2 |
| PF10_0161 | PHIST domain protein | ND | ND | 2 |
| Proteins identified as sumoylation substrates by single peptide | ||||
| PF11_0061* | Histone H4 | Chromatin structure | ND | |
| PF14_0334 | NAD(P)H-dependent glutamate synthase, putative | Glutamate metabolism | ND | |
| PFD0735c | Hypothetical Protein | ND | ND | |
| PF07_0030 | Heat shock protein 86 family | Heat shock protein | ND | |
| PFL0120c* | Cyclophilin, putative | Intracellular protein folding and signal transduction | ND | |
| PF11_0381 | Subtilisin-like protease2 | Invasion | Micronemes | |
| PF10_0055 | Hypothetical Protein | ND | ND | |
| PFE1120w | Hypothetical Protein | ND | ND | |
| PF11_0404* | Transcription factor with AP2 domain(s) | Transcription factor | ND | |
| PF14_0067 | LCCL domain-containing protein CCP3 | ND | ND | |
| PF14_0713 | RNA-binding protein/splicing factor | RNA binding/splicing | ND | |
| MAL7P1.68 | Zinc finger containing ERF2 subunit of a palmitoyltransferase | Palmitoylation | ND | |
| MAL8P1.7 | DNA-directed RNA polymerase II second largest subunit, putative | Transcription | ND | |
| PFF0975c | Conserved plasmodium protein | ND | ND | |
| PFF1440w* | SETdomain protein | Chromatin structure | ND | |
| PF10_0368* | Dynamin protein | Trafficking/organelle fission/ Cytoplasm, ER -Golgi, Apicoplast | ||
*
Orthologues known to be directly sumoylated and/or part of sumoylated complex.
ND, not determined; IE, infected erythrocyte.