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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Anal Chem. 2008 Jun 26;80(15):6093–6099. doi: 10.1021/ac702527b

Figure 2.

Figure 2

Trypsin digestion of BSA. (A) Different amounts of trypsin (5 μL) were added to 15 μL of solution containing 0.7 mM DTT and 4 μg of BSA in 10 mM NH4CO3: (1) 0.2, (2) 0.3, (3) 0.5, (4) 2, (5) 6, (6) 10 (7) 21 μM trypsin; (8) BSA was digested using conventional method of digestion; (9) no trypsin. Samples were incubated for 5 min at 52 °C. (B) Solution (20 μL) containing 0.5 mM DTT, 4 μg of BSA, and 0.5 μM trypsin in 10 mM NH4CO3 was incubated at 52 °C for different times: (1) 0, (2) 10, (3) 30, (4) 50 s, (5) 2, (6) 10, (7) 40 min;(8) 2, (9) 4 h. °C.