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Heterologous competition binding of 125I-labeled wild-type (1 nM) in the presence of increasing concentrations on nonlabeled CryIAb, N372A, N372G, DF-1, and D3 toxins. Gypsy moth BBMV (200 μg/ml per tube) was used, and each point is an average value of three individual experiments. Binding is expressed as a percentage of the total amount bound upon incubation with labeled toxin.
