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. Author manuscript; available in PMC: 2009 Jan 4.
Published in final edited form as: J Mol Biol. 2007 Nov 1;375(1):301–315. doi: 10.1016/j.jmb.2007.10.060

Figure 3.

Figure 3

Reduction of TA0289 by dithionite and reoxidation by air. 19 µM TA0289 in 50 mM Hepes-Na+ (pH 7.5), 0.2 M NaCl, and 10% glycerol (trace a) was scanned. A 50 mM stock of anaerobic sodium dithionite was then diluted 100-fold into the solution (to a final concentration of 500 µM), resulting in rapid reduction of TA0289 (trace b). After standing for several hours in air, a reoxidized spectrum (trace c) was obtained.