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. Author manuscript; available in PMC: 2009 Jan 4.
Published in final edited form as: J Mol Biol. 2007 Nov 1;375(1):301–315. doi: 10.1016/j.jmb.2007.10.060

Figure 6.

Figure 6

Reduction of cytochrome C by TA0289 in the presence of NorW and NADH. A 200 mg ml−1 sample of cytochrome C in 50 mM Hepes-Na (pH 7.5), 0.2 M NaCl, and 10% glycerol in the presence of 1 µM NorW and 1.9 µM TA0289 was scanned (trace a). Reduction of cytochrome C was achieved by the anaerobic addition of 200 µM NADH. The fully reduced spectrum is trace b. INSET: An absorbance trace at 550 nm following the time course for the reduction of 200 µg ml−1 cytochrome C in the presence of 200 µM NADH, 1 µM NorW, and 1.9 µM TA0289 in the same buffer, obtained from a single wavelength stopped flow experiment.