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. Author manuscript; available in PMC: 2009 Jan 4.

Published in final edited form as: J Mol Biol. 2007 Nov 1;375(1):301–315. doi: 10.1016/j.jmb.2007.10.060

Table 1.

Metal content of the recombinant CBS-Zn ribbon-like proteins and KTI11^a

Proteins and growth medium	Metal content (atoms/subunit)
Proteins and growth medium	Fe	Zn	Ni	Cu	Ca
TA0289 (TB^b)	0.18	0.74	n.d.^c	0.09	n.d.
TA0289 (M9^b)	0.67	0.17	n.d.	n.d.	n.d.
PH0267 (TB)	0.02	0.12	0.01	0.01	0.15
PF1953 (TB)	0.16	0.35	0.09	0.02	0.18
TV1335 (TB)	0.13	0.25	0.02	0.01	0.04
KTI11 (wt)^d (TB)	0.17	0.40	n.d.	0.01	0.04
KTI11 (wt) (M9+Fe²⁺)	0.49	0.03	n.d.	0.01	0.09
KTI11 (wt) (M9+Zn²⁺)	n.d.	0.97	n.d.	n.d.	0.09
KTI11 (C25S, C27S) (TB)	0.01	0.09	n.d.	0.03	n.d.
KTI11 (C47S, C50S) (TB)	0.03	0.03	0.01	n.d.	0.06
KTI11 (C25S, C27S, C47S, ΔC50) (TB)	0.01	0.02	0.02	n.d.	0.09

^a

Standard deviations for all determinations were 10.9 +/− 3.0%.

^b

TB, terrific broth; M9, minimal medium M9.

^c

n.d., not detected (below the detection limit).

^d

wt, wild type protein.