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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Neuropharmacology. 2008 May 21;55(2):198–203. doi: 10.1016/j.neuropharm.2008.05.010

Fig. 1. Acute cocaine treatment reduced the level of OFQ/N-IR in the rat midbrain.

Fig. 1

Rats were habituated to the motor activity tests, injected with saline or cocaine (20 mg/kg, i.p.) and sacrificed one hour later. Brain regions were isolated and homogenized in acid acetone solution by means of sonication. Samples were lyophilized, resuspended in RIA buffer and 50 µl of a 1:10 dilution of each sample were assayed in triplicates for the measurement of OFQ/N-IR using a commercially available RIA kit (Phoenix Pharmaceuticals; Belmont, CA). Data are mean (±SEM) of 6 rats/group. *indicates a significant reduction as compared to saline group (p<0.05).