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. Author manuscript; available in PMC: 2009 Oct 30.
Published in final edited form as: J Neurosci Methods. 2008 Aug 15;175(1):36–43. doi: 10.1016/j.jneumeth.2008.07.024

Fig. 5.

Fig. 5

Fig. 5

Fig. 5

Immunofluorescent localization of SMN in SMA fibroblasts treated with valproic acid (VPA) or hydroxyurea (HU). SMA patient fibroblasts (3813) were treated for 3 days with either 5 mM VPA (C), 65.7 µM HU (D) or vehicle (ddH2O; A). Fibroblasts were then labeled for SMN protein using 4F11. As a control, SMA carrier fibroblasts (3814; B) were also stained for SMN localization with 4F11. VPA and HU increase the amount of SMN in the nucleus and cytosol of patient fibroblasts. Arrowheads show SMN-positive gems in the nuclei. Scale bar, 10 µm.