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. Author manuscript; available in PMC: 2009 Oct 30.
Published in final edited form as: J Neurosci Methods. 2008 Aug 15;175(1):36–43. doi: 10.1016/j.jneumeth.2008.07.024

Fig. 7.

Fig. 7

Hydroxyurea (HU) increases human SMN protein in SMN2 transgenic mouse spinal cords. SMN2+/+;Smn+/− transgenic mice received a single intraperitoneal (i.p.) injection of HU (5 mg/kg) or vehicle (PBS). Six hours after injection, spinal cords were harvested and analyzed for human SMN protein using 4F11. Total SMN protein levels were shown using a pan-specific anti-SMN antibody. HU increases SMN2-derived SMN protein levels in the spinal cord. β-actin shows equal loading of protein in each lane.