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. Author manuscript; available in PMC: 2009 Jan 2.
Published in final edited form as: J Cell Sci. 2008 Oct 15;121(Pt 20):3487–3495. doi: 10.1242/jcs.016410

Fig. 8.

Fig. 8

SRp55 regulates VEGF splice-site selection in exon 8. (A) RPE cells were transfected with SRp55 or shRNA targeting SRP55, and VEGF165b expression measured by immunoblotting. siRNA targeting SRp55 downregulated both SRp55 and VEGF165b. (B) Densitometry indicated that both shRNAs targeting SRp55 inhibited SRp55 expression (n=4). (C) shRNAs targeting SRp55 also inhibited VEGF165b expression (n=3, *P<0.05, **P<0.01 compared with control, ANOVA, Dunnett’s test). (D) MS2-MBP assay for RNA-binding sites of HEK-cell nuclear extract. SRp55 was detected by immunoblotting in nuclear extract (NE) and when bound to a RNA construct containing the 35 nucleotides downstream of the stop codon for exon 8b (construct X), but not when this region was deleted (construct Y) or MS2 RNA (construct Z).