Table 2.

Subcutaneous infection of G. mellonella

			1:10		1:100		total
strain	tc-PAIYe	total number	dead	alive	dead	alive	dead	alive	dead [%] ± sd
Y. enterocolitica 2594	present2)	79	36	4	34	5	70	9	90 ± 9
Y. enterocolitica 4466	absent2)	96	47	1	34	14	81	15	88 ± 11
Y. mollaretii	present1)	64	30	2	24	8	54	10	84 ± 5
Y. bercovieri	absent1)	64	28	8	23	5	51	13	81 ± 11
Y. ruckeri	absent2)	85	33	11	20	21	53	32	53 ± 21
W22703-tcaA::Tn5 lux	present, but tcaA knockout	93	30	25	18	20	48	45	51 ± 13
W22703	present2)	114	32	25	20	37	52	62	41 ± 17
Y. aldovae	absent2)	68	23	13	4	28	27	41	41 ± 6
Y. kristensenii	absent2)	88	19	27	1	41	20	68	20 ± 12
controls
S. typhimurium	absent1)	88	10	33	10	35	20	68	18 ± 9
DH5α	absent1)	63	5	34	2	22	7	56	13 ± 6
LB		64					3	61	5 ± 0

G. mellonella larvae were subcutaneously infected with 5 μl of diluted overnight cultures. Strain W22703 and its tcaA knockout mutant confer similar toxicity towards the larvae (depicted in italics). Each experiment was independently performed at least three times, and the average dead rates are shown. Y. enterocolitica W22703: biotype 2, serotype O:9; Y. enterocolitica 2594: biotype 2, serotype O:9; Y. enterocolitica 4466: biotype 1B, serotype O:21. ¹⁾ according to the genome sequence, ²⁾ according to PCR analysis performed in this study.