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. 2009 Jan 6;7(1):e1000008. doi: 10.1371/journal.pbio.1000008

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© 2009 Skružný et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 4xGFP-Swt1 (green) or (B) 4xGFP-Swt1ΔPIN (green) were coexpressed with Nup120-mCherry (a marker for NPCs; red) in the indicated strains (swt1Δ, nup133Δ swt1Δ, nup133Δ swt1Δ + NUP133 [expressed from a centromeric plasmid], mlp1Δ swt1Δ, and nup60Δ swt1Δ) and analyzed by fluorescence microscopy. Cells were also viewed by Nomarski optics.