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. 2008 Dec 15;119(1):47–60. doi: 10.1172/JCI32310

Figure 2. IL-21 and IL-21R expression is upregulated after mechanical skin injury.

Figure 2

(A and B) Quantitative RT-PCR analysis of Il21 and Il21r mRNA expression in mouse skin following tape stripping (A) or application of acetone/dibutylphthalate to shaved skin (B). Results were normalized for Gapdh expression. (C) Time course of Il6 mRNA expression in tape-stripped skin. (D) FACS analysis of BMMCs for FcεRIα expression (open histogram). Filled histogram represents staining with isotype control. (E) RT-PCR analysis of Il21 mRNA expression in splenic CD4+ T cells and BMMCs. T cells were stimulated with P+I; BMMCs were stimulated with 0.25 μg/ml IgE anti-DNP plus 30 ng/ml DNP-HSA (cross-linking) or with 50 ng/ml TSLP for 24 h. Water was used as negative control. Data in A–C are expressed as fold induction compared with time point 0 h. Values are mean ± SD. *P < 0.05, **P < 0.01 versus 0 h. n = 5 per group.