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. 2008 Dec 22;119(1):169–181. doi: 10.1172/JCI35440

Figure 6. TIP30 forms cytoplasmic aggregates upon Contactin stimulation and increases susceptibility to cell death in vitro.

Figure 6

(A–E) A series of captured pictures from a time-lapse recording of EGFP-TIP30–transfected OLN-93 cells upon Contactin stimulation in vitro. TIP30, initially expressed diffusely in the cytoplasm, becomes confined to perinuclear areas within a few hours after Contactin stimulation. The original recording is available as Supplemental Video 1. (F and G) Photographs at higher magnification of prestimulation (0 minutes) and post-Contactin stimulation (240 minutes) from A–E, showing perinuclear cytoplasmic aggregations of TIP30 upon Contactin stimulation. (H–M) In those cells with relatively high expressions of TIP30, perinuclear aggregations of TIP30 upon Contactin stimulation resulted in breakdown of the nuclear envelope and subsequent cell death (as seen in M) within several hours. The original recording is available as Supplemental Video 2 (nontransgenic control is shown in Supplemental Video 3). Scale bars: 40 μm (A–E); 20 μm (F–M).