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. Author manuscript; available in PMC: 2009 Jan 4.
Published in final edited form as: Nat Med. 2000 Jan;6(1):86–90. doi: 10.1038/71582

Fig. 2.

Fig. 2

Adhesion of P. falciparum-infected erythrocytes to HA and CSA. a, Adhesion of IEs is abolished by pre-treatment of immobilized HA (■) and CSA (□) with testicular hyaluronidase but not heparinase. Treatment with chondroitinase ABC reduces adhesion to CSA to a much greater degree than to HA. b, Soluble HA (■) but not colominic acid (□) added to IE suspensions before the adhesion assays competitively inhibits binding to immobilized HA in a concentration-dependent manner. There is some inhibition with soluble CSA (◆). c, Adhesion of IEs to immobilized HA (■) is effectively inhibited by oligosaccharide fragments of HA of dodecamer (12-mer) size or larger. There is no substantial inhibition of adhesion to CSA (□) (only the results for the hexadecamer (16-mer) fragments are shown). d, Surface proteins on intact IEs were cleaved with trypsin before adhesion to each of HA and CSA was tested. Trypsin treatment abolishes adhesion to immobilized HA (■) but not to CSA (□). All data represent the proportion of bound IEs expressed as a percentage of control (mean ± s.e.m. for multiple experiments).