Figure 5.

Preincubation of ruby-eye mouse mast cells with an extracellular saline containing 10 μM serotonin restores the early secretory response. After 1 h of incubation, the mast cell was patch-clamped with a pipette solution containing 5 μM GTPγS. As in Fig. 4, the upper recordings correspond to the membrane capacitance and the lower traces are the amperometric recordings. The dotted line corresponds to the integral of the amperometric trace.