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DiY in SPE-treated urines was separated by HPLC and then detected using a mass spectrometer. The sample preparation was done as described in the Materials and Methods section. The analysis of the mass spectra was done by “Method I”. The final concentration of the internal standards cocktail including [13C18]DiY is 50 nM. The MRMs of 361.2/315.1 and 361.2/237.1 show the native DiY and those of 379.2/332.1 and 379.2/253.1 derived from the stable internal standard of DiY.
