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. 2009 Jan 12;4(1):e4174. doi: 10.1371/journal.pone.0004174

Figure 1. Specificity of the anti CK1δ polyclonal rabbit serum NC10.

Figure 1

An immunoabsorption test for NC10 was used to show its specificity in immunohistochemistry (IHC). Immersion fixation with acetic formalin, alkaline phosphatase reaction, dye: newfuchsin. IHC was performed on paraffin-embedded pancreatic tissue of a 5 week old BALB/c mouse using NC10 (A) or NC10 preincubated with either a control peptide (the p53 specific peptide MEESQSDISLELGGC, 0.1 µg (B)) or with the specific blocking peptide used for immunisation of rabbits (CGDMASLRLHAARQGARC, 0.1 µg) for 3 h at 4°C (C). The results indicate that the antigenic peptide, but not the control peptide competively inhibits CK1δ binding. Magnification: 400×.