Table 3. Effect of different blocking reagents on background reduction of CK1δ immunostained frozen sections.
blocking solution | specific against | background reduction |
Levamisole | endogenous alkaline phosphatase | + + |
peroxidase-blocking reagens (DAKO) | endogenous peroxidase | + + |
Uni-Block (BioGenex) | endogenous biotin | + + + |
Avidin/Biotin Blocking Kit (Zymed) | + | |
blocking solution | unspecific against | background reduction |
Aurion BSA-c, (Biotrend) | hydrophobic and electrostatic interactions | − |
Power Block, Casein (BioGenex) | − | |
anti- IgG F(ab′)2-fragment | background reduction | |
• Anti-Rabbit IgG F(ab′)2 biotinylated (DAKO) | − |
Frozen tissue sections were immunostained for CK1δ in the presence and absence of the indicated blocking reagens. − no effect of the blocking reagens; + low background reduction; ++ medium background reduction; +++ strong background reduction.