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. 2009 Jan 12;4(1):e4173. doi: 10.1371/journal.pone.0004173

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Mabilleau et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The number of multinucleated TRAcP positive cells formed in response to IL-32/sRANKL treatment was significantly reduced in the presence of excess OPG. (B) The size of the newly-formed TRAcP positive multinucleated cells was unaffected by the presence of OPG in IL-32/sRANKL-treated cultures. (C) The number of nuclei noted in TRAcP positive cells formed in response to IL-32/sRANKL treatment was not affected by the OPG treatment. (D) The percentage area lacunar resorption was completely abolished when PBMCs were treated with IL-32/sRANKL and excess OPG. P values represent the statistical significances between each group using Mann-Whitney test.