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. 2009 Jan 13;4(1):e4183. doi: 10.1371/journal.pone.0004183

Figure 6. h-S100A7 adenoviral infection in primary cortico-hippocampal neuron cultures derived from Tg 2576 embryos coincides with activation of p42/44MAPK and PKC signaling.

Figure 6

A,B, cortico-hippocampal neuron cultures derived from Tg2576 embryos were infected with LacZ or h-S100A7 adenovirus for 24 hours at 10 MOI. The phosphorylation of p42/44 MAPK and PKC were analyzed in cell lysate and detected by pTpY185/187-p42/44 MAPK antibody or total p42/44 MAPK antibody (A) and phosphor-PKC (pan) antibody or PKCα antibody (B). In this study, tissue-culture dishes were placed on ice (4°C) and, following the removal of conditioned media, cells were immediately collected in ice-cold 1× SDS-sample buffer containing protease inhibitors. Data represent mean±SEM of determinations made in three separate cultures; n = 3 to 4 per culture; two-tailed Dunnett's t-test vs. LacZ infection: *P<0.05, two-way t-test.