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SLO mediates activation of effector caspases-3 and -7. A, apoptotic caspase activity (DEVDase) in cell lysates of macrophages infected with WT GAS strains, but not the ΔSLO mutant as assessed by cleavage of fluorescent substrate Ac-DEVD-AFC. B, SLO expression in L. lactis is sufficient to induce apoptotic caspase activity in macrophage lysates.
