FIGURE 1.

Direct interactions of purified Fhit forms with Hsp60/10 and Fdxr. A, immunoblot of preparations of purified GST-tagged proteins for in vitro interaction studies. Examples of the purified proteins made in bacteria are shown. B–D, interactions between purified Fhit and interactor proteins. GST-tagged or ³⁵S-labeled proteins made by in vitro transcription-translation were prepared and combined in pairs as indicated, followed by radiographic imaging of pulled down or immunoprecipitated partner proteins. E, interaction of purified Fhit and phospho-Fhit forms with effectors. Purified Fhit and phospho-Fhit forms were combined with labeled interactors; IP was with anti-Fhit. The upper panel shows the radiographic image of co-IP of Hsp60 and the lower panel co-IP of Fdxr with the Fhit forms. F, anti-Fhit immunoprecipitates phospho-Fhit monomers. This panel shows the immunoblotted Fhit proteins precipitated for each of the IP experiments shown in E; the anti-Fhit serum precipitates and detects phosphorylated and unphosphorylated forms of Fhit.