FIGURE 6.

Cell cycle kinetics of H₂O₂-treated MKN74/Fhit and Fhit mutant expressors. MKN74 stable Fhit or mutant-expressing cells were treated with 0.25 mm H₂O₂ and incubated for 24 (top panels) or 48 h (bottom panels). Cells were collected, washed with PBS, and resuspended in cold 70% ethanol. For analysis, cells were spun down, washed in PBS, and suspended in 0.1 mg/ml propidium iodide/Triton X-100 staining solution (0.1% Triton X-100, 0.2 μg/ml DNase-free RNase A) for 30 min at room temperature and analyzed by flow cytometry.