FIGURE 7.
Tumorigenicity of MKN74/Fhit and Fhit mutant expressors in nude mice. 5 × 106 WT or mutant (Y114A, Y114D, Y114F, H96N, and L25W) stable expressing MKN74 cells were injected subcutaneously into the right flanks of four nude mice for each cell line. Tumor sizes were measured over a 2-week period and volumes of tumors calculated and graphed with standard deviations as shown. Significant differences in pairwise comparisons of tumor sizes of different MKN74/Fhit and mutant expressors were determined by Student's t test. The tumors from MKN74/Fhit WT cells were significantly smaller than tumors of all mutant expressors (values ranging from p = 0.008 for Y114A to p = 0.04 for Y114D and L25W) except H96N (p = 0.2). The MKN74/H96N tumors were significantly smaller than Y114A (p = 0.01) and Y114F (p = 0.02) tumors. There were no other significant differences.