TABLE 2.

Steady-state kinetic constants for wild-type and mutant BT1713-catalyzed hydrolysis of KDN-9 phosphate in 50 mm K⁺ HEPES containing 2 mm MgCl₂ (pH 7.0, 25 °C)

BT1713	kcat	Km	kcat/Km
	s−1	mm	m−1s−1
Wild type	1.2 ± 0.1	0.10 ± 0.01	1 × 104
R64A	<10−4
E56A	0.026 ± 0.002	0.41 ± 0.01	6 × 101
S37A	1.2 ± 0.1	0.20 ± 0.01	6 × 103
T34A	0.09 ± 0.01	0.18 ± 0.01	5 × 102