TABLE 1.
Relative fluorescence intensity of CD44 on cells and protein-coated beads
CD44 expression on cells or microspheres coated with immunoprecipitated CD44 from the corresponding cell type was quantified by single-color immunofluorescence and flow cytometry using the PE-conjugated anti-CD44 mAb 515. Background levels were determined by incubating cell or microsphere suspensions with the properly matched PE-conjugated mouse IgG isotype control antibody. Values represent mean fluorescence intensity ± S.E. from n = 5 to 8 experiments.
Cell type
|
Mean fluorescence intensity
|
|
---|---|---|
Cells | Beads | |
LS174T | 1205 ± 88 | 1191 ± 139 |
HL60 | 626 ± 10 | 700 ± 66 |
T84 | 654 ± 68 | 726 ± 195 |