FIGURE 5.

Met transit from early endosomes to the MVB is delayed in response to LRR. HeLa cells were allowed to internalize Alexa⁴⁸⁸-labeled InlB or LRR for 15 min at 37 °C and then chased at 37 °C in the absence of ligand for increasing times to promote ligand transport from early endosomes to the MVB. Cells were fixed and stained for endogenous EEA1 or CD63, and the relative amount of internalized ligand that co-localizes with these proteins was quantified using confocal microscopy. Values are expressed as the percentage of co-localization ± S.E. normalized to an area from three independent experiments (*, p < 0.01, ANOVA).