FIGURE 2.
Effect of Spry2 down-regulation on the regulation of p85, Akt, HDM2, and p53. A, the indicated cell strains were deprived of serum for 12 h and then treated with EGF (100 ng/ml) for the indicated times (in min). Whole cell lysates (WCL) were prepared and assayed by Western blotting to determine the levels of the indicated proteins; pp85, phospho-85; pAkt, phospho-Akt; pHDM2, phospho-HDM2. B, bar graphs showing the quantification of the bands in the Western blot in panel A. C, the cell lines were exposed to UV radiation (90 J/m2) and allowed to grow under normal conditions for the indicated time periods (in hours) following UV treatment. WCL were prepared and then analyzed by Western blotting with the indicated antibodies. D, bar graphs showing the quantification of the bands in panel C.