TABLE 1.
Specific activity of recombinant chondroitinase ABC II on glycosaminoglycan substrates Specific activity was determined by monitoring the increase in absorbance at 232 nm for 5 min. The initial rate of increase in A232 nm was determined for each substrate. The enzyme activity in units (1 unit = 1 μmol product formed/min) was calculated from the initial rate using ε = 3800 m–1 for reaction products at pH 8.0. ND, not determined.
| Substrate | Specific activity |
|---|---|
| milliunits/mg protein | |
| Chondroitin-6-sulfate | 29,000 |
| Chondroitin-4-sulfate | 18,000 |
| Dermatan sulfate | 17,000 |
| Chondroitin | 5400 |
| Chondroitin sulfate D | 4900 |
| Chondroitin sulfate E | 4900 |
| Hyaluronan | ND |
| Heparin/heparan sulfate | ND |
| Keratan sulfate | ND |