TABLE 1.
Exon D is required to interact with Rab10. The table illustrates that only those myosin V tails that contain exon D were able to react with the Rab10 bait in a blue/white yeast two-hybrid assay. In addition, all of the myosin Va and myosin Vb tails tested were able to interact with Rab11a and Rab11-FIP2 regardless of alternate exon usage. Rab8a interacted with myosin Va tails with exon D (ABCDEF, ACDEF, and ABCDE), with myosin Vb tail without exon D (ABCE), and with myosin Vc tail (which expresses exon D) but did not interact with myosin Va tails lacking exon D (ABCE and ACE) and myosin Vb tail with exon D (ABCDE). Similar to the results for the full tail segments, GAL4 fusion constructs expressing only exons A, B, C, D, and E of myosin Vb (lacking the globular tail domain) interacted with Rab10, but not Rab8a, whereas exons A, B, C, and E interacted with Rab8a but not Rab10. Neither set of exons interacted with Rab11a or Rab11-FIP2.
| pAD- | pBD-Rab8a | pBD-Rab10 | pBD-Rab11a | pBD-Rab11-FIP2 |
|---|---|---|---|---|
| Myosin Va tail (ABCDEF) | + | + | + | + |
| Myosin Va tail (ACDEF) | + | + | + | + |
| Myosin Va tail (ABCDE) | + | + | + | + |
| Myosin Va tail (ABCE) | − | − | + | + |
| Myosin Va tail (ACE) | − | − | + | + |
| Myosin Vb tail (ABCDE) | − | + | + | + |
| Myosin Vb tail (ABCE) | − | − | + | + |
| Myosin Vc tail | + | + | − | − |
| Myosin Vb Exons ABCDE | − | +/− | − | − |
| Myosin Vb Exons ABCE | + | − | − | − |