TABLE 2.
k2/Ks values of [14C]penicillin G for PBP 2 variants
| PBP2 variants | k2/Ks | Fold decrease |
|---|---|---|
| m−1s−1 | ||
| WT (n = 14)a | 63,700 ± 5,500 | |
| 6140 (n = 10) | 4,000 ± 2,000 | 15.9 |
| D345a (n = 8) | 12,200 ± 2,400 | 5.2 |
| 6140CT (n = 8) | 12,400 ± 900 | 5.1 |
| F504L (n = 6) | 35,500 ± 4,100 | 1.8 |
| A510V (n = 3) | 50,200 ± 2,700 | 1.3 |
| A516G (n = 5) | 44,700 ± 3,000 | 1.4 |
| P551S (n = 12) | 21,300 ± 800 | 3.0 |
| P551S/F504L (n = 7) | 13,100 ± 700 | 4.9 |
| P551S/A510V (n = 3) | 18,800 ± 60 | 3.4 |
| P551S/A516G (n = 6) | 25,900 ± 2,400 | 2.5 |
a
The number of determinations of k2/Ks for each construct.