FIGURE 4.
Salubrinal is a nontoxic mediator of protection against oxidative glutamate toxicity in immature cortical neurons. 1 × 105 primary cortical neurons in 96-well plates were grown for 24 h with (Salu) or without (Ctrl) 30 μm salubrinal and then treated with glutamate at the indicated concentrations or without glutamate for another 24 h followed by an MTT assay. A, viability in the absence of glutamate and salubrinal was normalized to 100%. B, viability without glutamate with or without salubrinal was normalized to 100%. Graphs represent means of four independent experiments each done in triplicate. Statistical analysis was performed by one-sample t test compared with 100 (A) or two-way ANOVA (B). Viability of cultures treated with salubrinal alone was not different from control cells, whereas cells treated with salubrinal were significantly more resistant to glutamate (column factor, p < 0.0001).