FIGURE 6.

Platelets release chemerin upon activation. A, ELISA quantification of total chemerin present in resting and thrombin (5 units/ml) activated PRP. B, in vitro transwell chemotaxis of CMKLR1/L1.2 cells to PRP, and PRP/thrombin (5 units/ml), PPP, and PPP/thrombin (5 units/ml). Thrombin at 5 units/ml was added to PRP or PPP at 37 °C for 5 min. C and D, identification of chemerin in washed platelet lysates by Western blot analysis (C) or in vitro transwell chemotaxis of CMKLR1/L1.2 cells (D). The platelets were treated with the indicated platelet-activating agonists at 37 °C for 3 min: thrombin receptor-activating peptide (20 μm), thrombin (5 units/ml), collagen (10 μg/ml), and ADP (10 μm). 200 μl of platelet releasates were tested in chemotaxis assays. The results represent one of three independent experiments and are expressed as the means ± S.D. (n = 3). **, p < 0.005.