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The correlation between iron- and ED1-positive cell staining in a POD 106 allograft. Optical micrograph (magnification ×200) of 2 neighboring 5-μm sections stained with Perl's Prussian blue for iron (A, blue, arrows) and anti-rat ED1-positive mAb for monocytes/macrophages (B, brown, arrows). The distribution of the iron-positive cells shown correlates well with ED1-positive cells in C by overlay image of A and B.
