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. Author manuscript; available in PMC: 2009 Nov 27.
Published in final edited form as: Nature. 2008 Oct 19;456(7221):524–528. doi: 10.1038/nature07433

Figure 1. Requirement for 53BP1 in NHEJ of dysfunctional telomeres, but not DNA damage signaling.

Figure 1

a, (Top) Metaphase chromosomes after deletion of TRF2 from the indicated cells. Telomeric FISH, green; DAPI, red. (Bottom) Summary of the effect of 53BP1 on telomere fusions at 120 hours after TRF2 deletion.

b, In-gel assay for the 3’ overhang (left) and total telomeric DNA (right) after deletion of TRF2 from 53BP1-proficient and -deficient cells.

c, Quantification of overhang signals in (b) (mean±SD; n=3).

d, IF for presence of γ-H2AX at telomeres after deletion of TRF2 from 53BP1-proficent and -deficient cells.

e, Immunoblots for phosphorylated ATM and Chk2 after TRF2 deletion.

f, Proliferation of MEFs of the indicated genotype and treatment (mean±SD; n=3).