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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Orthop Res. 2008 Dec;26(12):1577–1584. doi: 10.1002/jor.20714

Figure 1. Cytosolic induction and nuclear accumulation and activation of NFAT in response to PMMA particles.

Figure 1

A) Cytosolic and nuclear protein extracts were isolated from bone marrow macrophages after treatment with the control media or PMMA (0.1mg/ml) for the time points indicated. Equal amounts of extract protein were electrophoresed, transferred to a nitrocellulose membrane and analyzed by immunoblotting with anti-NFAT2, beta-actin, and histone-1 antibodies. B) Osteoclast precursor cells were cultured and treated with control and PMMA (0.1mg/ml). Cells were lysed 1 hour and 24 hours after PMMA treatment and were subjected to electrophoretic mobility shift assay (EMSA) using radiolabeled NFAT consensus oligonucleotide.