Figure 1.

FAA in Mc3r−/− mice is significantly attenuated compared with WT mice. A, Averaged wheel running actograms for WT (left) and Mc3r−/− (right) mice subjected to 4 h of RF. Periods during which food was available are shaded. B, Double plotted graphs of average wheel turns for 9 d (day 4 to day 13) for WT and Mc3r−/− mice during AL (top) or RF conditions (bottom). C, Daily FAA during the 3 h before food presentation. Significant differences (*) between WT and Mc3r−/− mice were observed on day 2 (p < 0.05), day 4, day 6 (both p < 0.01), and day 9 (p < 0.05). D, FAA adjusted for 24 h total activity. Significant differences (*) between WT and Mc3r−/− mice were observed on day 2, days 5–8, day 10 (all p < 0.01), and day 12 (p < 0.05). E, Weight loss during RF was minimized to ∼10% of pre-RF weight. Black bar represents wheel turn responses during RF after weight stabilization.