Figure 7.

Model for the Transcriptional Regulation of PR-10a by the Transcription Factors Why1, SEBF, and Pti4.

(A) In uninduced tissues, the repressosome SEBF-Pti4 is recruited to the SE through the cs-RBDII of SEBF, while the cs-RBDI and potentially the acidic domain (Ac) of SEBF are required to interface with the ERF domain of Pti4. Recruitment of the repressosome to PR-10a prevents transcription through a yet to be identified mechanism. Bas and Gly indicate a basic domain and a Gly-rich region, respectively. Their functions are unknown. TATA refers to the TATA box.

(B) In induced tissues, the SEBF-Pti4 repressosome is dismissed from the PR-10a promoter, while the ERE is populated by the transcriptional activator Why1, which is a mandatory step in the transcriptional activation of PR-10a. According to the accepted gene activation paradigm, Why1 would contact, directly or indirectly, the basal transcription machinery.

In both panels, the numbers indicate the nucleotide positions in the promoter with respect to the RNA start site.