Figure 2.

Involvement of SA in the erh1-Mediated Cell Death Phenotype.

(A) The erh1 mutation results in SA accumulation. Leaves of 6-week-old plants of the indicated genotypes were assayed for free and total SA. The amount of conjugated SA is the subtraction of free SA from total SA. Data represent means ± se from three duplicate samples in one experiment. Asterisks indicate that the value of the erh1 mutant is significantly different (P < 0.01) from that of the respective wild-type lines based on Student's t test. This experiment was repeated twice with similar results.

(B) erh1-activated SHL is SA-dependent. erh1 and erh1 plus RPW8 (from line S5) were introduced into the NahG background by crossing, and respective F3 lines homozygous for erh1 or erh1/RPW8 and NahG from the respective crosses were examined for SHL by trypan blue staining at 5 weeks old. [See online article for color version of this figure.]