Figure 6.

Additive/Synergistic Effects of acd5 and erh1 on Cell Death.

(A) acd5 has enhanced resistance to powdery mildew. Five-week-old plants were inoculated with G. cichoracearum UCSC1, and disease phenotypes at 8 dpi were shown by two representative leaves for each genotype. Powdery mildew infection can be seen as white powdery coating on Col-0 leaves.

(B) Six-week-old, short-day-grown plants of the indicated genotypes. Note the leaf cell death and reduced plant stature of acd5/S5.

(C) Four-week-old, short-day-grown plants of the indicated genotypes. Note the leaf cell death and reduced plant stature of erh1/acd5.

(D) Two-week-old, short-day-grown plants of the indicated genotypes. Note the collapsed cotyledons and stunted growth of erh1/acd5/S5.

(E) Silencing a rice ERH1 homolog leads to cell death and reduced plant stature. Two-and-one-half-months-old wild-type Nipponbare rice and one representative rice line transgenic for an RNAi construct targeting a rice ERH1 homolog (Os01g0850100) are shown. Arrows indicate dead leaves.

(F) RT-PCR analysis of the indicated genes from four rice transgenic lines along with the parental wild type.