Table 10.

Complementation of lre-3 by pC450 and pCLRE

	lre-3/lre-3; pC450	lre-3/lre-3; pCLRE-1	lre-3/lre-3; pCLRE-3
Normal embryo sacs	246 (89.13%)	161 (85.63%)	206 (81.74%)
Invasive pollen tube	29 (2.42%)	11 (5.85%)	31 (12.30%)
No pollen tube	4 (1.45%)	31 (12.30%)	15 (5.95%)

lre-3 homozygous plants were transformed by pC450 carrying the annotated At4g26450 gene or pCLRE, a binary vector carrying the lorelei genomic sequence. Complementation was assayed by scoring the lre phenotype 48 h after pollination using aniline blue staining.