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. 2008 Nov;20(11):3038–3049. doi: 10.1105/tpc.108.061713

Table 8.

Complementation of lre-2/+ by the pC450 Construct

Kanr Kans Ratio Kanr/Kans
lre-2/+; pC450.6-2 235 (67.0%) 116 (33.0%) 2.02:1
lre-2/+; pC450.6-3 155 (51.8%) 144 (48.2%) 1.08:1
lre-2/+; pC450.7-4 249 (66.6%) 125 (33.4%) 1.99:1
lre/-2+; pC450.7-7 255 (66.4%) 129 (33.6%) 1.98:1

lre-2/+ plants were transformed with the binary vector pC450 carrying the annotated At4g26450 gene. Complementation was confirmed in several independent transformed lines by checking the segregation of the nptII gene conferring kanamycin resistance (Kanr) linked to the lre-2 mutation. Kans, kanamycin sensitivity.