Figure 1.
HAB1 and SWI3B Interact in a Yeast Two-Hybrid Assay.
Interaction was determined by growth assay on medium lacking His and adenine. Dilutions (10−1, 10−2, and 10−3) of saturated cultures were spotted onto the plates.
(A) Top, interaction assay with ΔNHAB1 as bait (fused to the GBD) and either full-length or different deletions of SWI3B as putative preys (fused to the GAD). Schemes of SWI3B domains and the different protein deletions are shown. Deletions C1 and C2 lacked C-terminal amino acid residues 346 to 469 and 221 to 469, respectively. Deletion N1 lacked N-terminal amino acid residues 1 to 220. GAD-SWIRM and GAD-ZZ comprised amino acid residues 1 to 140 and 134 to 220, respectively. Middle, interaction assay with SWI3A, SWI3B, SWI3C, and SWI3D as putative preys. Bottom, interaction assay with ΔNPP2CA, ΔNABI1, and ΔNABI2 as baits and SWI3B as prey.
(B) Protein phosphatase activity of MBP-HAB1, MBP-ΔNHAB1, and MBP-G246D ΔNhab1 fusion proteins. Values are averages ± se from three independent experiments.
(C) Interaction assay with ΔNHAB1 and G246D ΔNhab1 as baits and SWI3B as prey.