FIG. 5.

PSG23N1A induces TGFB1 and VEGFA in human monocytes and trophoblast cells. A, B) Human monocytes were treated with 30 μg/ml PSG23N1A for 24 h. Statistical significance between protein-treated and untreated samples was determined by two-tailed Student t-test, and * indicates P < 0.05. C, D) HTR-8/SVneo cells were treated with PSG23N1A in concentrations of 10–30 μg/ml for 24 h. Statistical significance between doses was determined by one-way ANOVA, and the P value between doses was <0.001. TGFB1 and VEGFA in the supernatants were analyzed by ELISA. All cells were treated in triplicate, and data is representative of at least three independent experiments. Error bars represent mean ± SEM.