Table III.
Relative specific activities of anise AIMT1 and Clarkia IEMT with t-anol, isoeugenol, and a variety of related substrates
All substrates were tested at 10 μm of a final concentration, since some of the substrates are not soluble in aqueous solutions at higher concentrations.
| Substrates | Relative Activity (%) for Each Enzyme Assayed
|
|
|---|---|---|
| AIMT1 | IEMT | |
| Chavicol | 5.9 ± 7.1a | NDb |
| t-Anol | 45.3 ± 18.1 | ND |
| Dihydrochavicol | 22.8 ± 3.8 | ND |
| Eugenol | 9.1 ± 3.8 | 108.3 ± 1.7 |
| Isoeugenol | 100.0c | 100.0c |
| Dihydroeugenol | 48.5 ± 3.8 | 211.1 ± 10.6 |
| 5-Methoxyeugenol | 17.8 ± 4.8 | 115.0 ± 4.5 |
a
Values are averages of three independent experiments ±se.
b
ND, No activity detected.
c
Specific activities for AIMT1 and IEMT were 5.83 and 1.30 pkat/mg protein, respectively.