Table 2.

In vivo activation of wild-type and mutant PhoB proteins

Regulator	By ′PhoR*		By ′VanS†
	Bap sp act	Relative to PhoBwt	Bap sp act	Relative to PhoBwt
PhoBwt	186  ±  13	1.0	0.3  ±  0.0	1.0
PhoBM17V,E87D	469  ±  10	2.5	55.0  ±  15.9	167
PhoBE87D	207  ±  25	1.1	1.7  ±  0.2	5.2
PhoBM17V	410  ±  20	2.2	0.5  ±  0.1	1.6
PhoBT97A	182  ±  33	1.0	142  ±  4	432
PhoBQ28L,T83S,S138R	236  ±  2	1.3	128  ±  11	389
PhoBT83S	217  ±  21	1.2	137  ±  6	416

Cells were grown with arabinose and assayed as in Table 1. Each phoB allele is in single copy in a pSK58 or pAH85 derivative. Bap, bacterial alkaline phosphatase; sp act, specific activity. 

^*Integrants of BW23423 were assayed. No arabinose values were 0.3 ± 0.0 or less. 

^†Integrants of BW23425 were assayed. No arabinose values were 0.6 ± 0.0 or less.